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KMID : 0361319840160010030
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Korean Journal of Medical Technologists
1984 Volume.16 No. 1 p.30 ~ p.34
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Evaluation of Serum Bilirubin by Artificial Bilirubin Standard
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Abstract
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Bilirubin in serum exists in two forms; one relatively water soluble, reacts readily in aqueous solution called direct bilirubin or conjugated bilirubin.
The other form reacts very slowly unless a solubilizing agent is used and is termed as indirect or unconjugated bilirubin.
The ratio of conjugated to unconjugated bilirubin may vary in different types of liver diseases-Most methods for the determination of bilirubin are based on the diazo reaction in which azobilirubin is formed.
The Evelyn-Malloy method, which is widely used, has been almost standard method. Direct bilirubin is determined by photometric measurement of the color developed exactly one minute after diazotization.
Methanol is then added which releases indirect bilirubin into solution permitting it to undergo diazotization. The final color developed represents total bilirubin.
Bilirubin (Bilirubin standard) is stable for approximately 24 to 48 hours at room temperature if kept in total darkness.
When exposed to white light, either sunlight or artificial, direct and indirect bilirubin undergo photo-oxidation or dehydrogenation to biliverdin some itermidiate product.
So we attempt to introduce and establish more stable bilirubin determination method using a artificial bilirubin standard (Pontacyl viloet 6R solution).
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